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KMID : 0385520100230060524
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Analytical Science & Technology
2010 Volume.23 No. 6 p.524 ~ p.530
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Accurate determination of chloramphenicol in meat by isotope dilution liquid chromatography mass spectrometry (ID-LC/MS)
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Song Hye-Min
Kim Byung-Joo
Jeong Hyuk
Ahn Seong-Hee
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Abstract
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Chloramphenicol is one of the most effective antibiotics for treatment of food-producing animals for typhoid fever. However, it has been reported that it caused severe side effects such as aplastic anemia in human, therefore the use of chloramphenicol for food-producing animal is prohibited by European Union and other countries. In this study, the analytical method using isotope dilution liquid chromatography-mass spectrometry (ID-LC/MS) was established for accurate determination of chloramphenicol in meat. Chloramphenicol was extracted with ethylacetate from porcine and solid phase extraction cartridge was used for enhancing the recovery. The residue of chloramphenicol in porcine was analyzed using the liquid chromatography mass spectrometer (LC/MS) interfaced with electrospray ionization source. Analysis was performed in negative mode with selected reaction mornitoring mode at m/z 321→257 of [M-H]?→[M-H-(HCOCl)]?and m/z 326→262 channels for its isotope. The established method was tested using fortified samples at the level of 0.2 1, 10, 25 ㎍/㎏ and analytical results agreed with the gravimetrically fortified values within their uncertainties. This method was validated by analyzing a certified reference material, BCR 445, from IRMM (Institute for Reference Materials and Measurement). Our measurement values agreed with the certified value within their uncertainties. The uncertainty of our measured value was much lower than that the certified value from IRMM.
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KEYWORD
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chloramphenicol, ID-LC/MS/MS, selected reaction monitoring(SRM)
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